Effect of Isatin on Ischemia and Reperfusion Injury: an Experimental Study in the Isolated Rat Heart
Zuhal Guksu, Orkide Palabıyık, Aziz Karaca, Nejdet Süt, Selma Arzu Vardar
Keywords: Isatin, atrial natriuretic peptide, ischemia, cGMP
Introduction: Isatin is an endogen indole that is found in body fluids and reported as a neuroprotective, anticonvulsant, and sedative agent. The aim of the present study was to investigate the effects of isatin on left ventricle functions before and after low-flow ischemia in the isolated rat heart. Patients and Methods: Male Wistar rats were divided into four groups. Isatin (I, 50 mg/kg intraperitoneally) was administered in the I and I-atrial natriuretic peptide (ANP) groups 20 min before the hearts were placed on the Langendorff apparatus. Serum physiologic was administered to the control (C) and ANP groups. Low-flow ischemia was applied in all groups. ANP (0.1 μM/L) was added to perfusion solution in the ANP and I + ANP groups 15 min before ischemia. Left ventricular developed pressure (LVDP) and maximum and minimum pressure changes were recorded before and after ischemia. Cyclic guanosine monophosphate (cGMP) levels were measured in the perfusate in all groups. Results: LVDP and minimum pressure change values of the I group were found to be similar to the C group at 60 min of reperfusion. The level of cGMP in the I group was similar to the C group but lower than the ANP and I + ANP groups before and after ischemia. Conclusion: The administration of isatin prior to cardiac ischemia does not significantly alter cardiac function during the reperfusion period in rat heart. The results of the present study showed that isatin may not appear to have a disturbing effect on cardiac functions after low-flow ischemia.