Orkide Palabıyık1, Muhammed Ali Aydın2, Ecem Büşra Değer2, Selçuk Korkmaz3, Selma Arzu Vardar2

1Department of Medical Services and Techniques, Trakya University Vocational School of Health Services, Edirne, Türkiye
2Department of Physiology, Trakya University Faculty of Medicine, Edirne, Türkiye
3Department of Biostatistics and Medical Informatics, Trakya University Faculty of Medicine, Edirne, Türkiye

Keywords: Fructose; ischemia; reperfusion; heart


Introduction: This study aimed to investigate the effects of fructose on cardiac hemodynamics and infarct size and the role of the antioxidant mechanism in these effects in isolated rat hearts undergoing ischemiareperfusion.

Patients and Methods: Isolated hearts obtained from female Wistar rats were perfused with Krebs-Henseleit solution containing 12 mM glucose or solution containing 12 mM fructose or 48 mM fructose and underwent lowflow ischemia followed by reperfusion on the Langendorff apparatus. Left ventricular developed pressure (LVDP), timedependent pressure changes (dp/dt max, dp/dt min) and heart rates were recorded at the 1st, 15th and 120th minutes of reperfusion following ischemia, and the percentage of the infarct area and the size of the risk area were determined. At the end of the reperfusion, total oxidant capacity (TOS), malondialdehyde (MDA) and glutathione (GSH) levels were examined in perfusion fluid samples.

Results: Basal dp/dt max values were lower in the high fructose group compared to the glucose group (p= 0.032). When the hearts were perfused with 12 mM fructose, a significant increase was observed in the percentage of the ischemic area and risk area compared to equivalent glucose and high fructose (p< 0.001 and p< 0.001, respectively). MDA, GSH and TOS values were comparable in all groups.

Conclusion: The present study shows that fructose perfusion plays a role in reduced ventricular contractile function relative to glucose in isolated rat hearts. This reduction triggered by fructose appears to be independent of antioxidant mechanisms. Furthermore, fructose perfusion at glucose-equivalent concentration causes a greater increase in infarct area in ischemic hearts, whereas an increase in fructose concentration appears to prevent this effect.

Cite this article as: Palabıyık O, Aydın MA, Değer EB, Korkmaz S, Vardar SA. The acute effect of fructose on cardiac hemodynamic responses and infarcted area in isolated rat heart during ischemia-reperfusion. Koşuyolu Heart J 2023;26(1):7-13.

Ethics Committee Approval

The approval for this study was obtained from Trakya University Local Ethics Committee of Animal Experiments (Decision no: 2021.05.05, Date: 28.05.2021).

Peer Review

Externally peer-reviewed.

Author Contributions

Concept/Design - OP, MAA, EBD, SAV; Analysis/Interpretation - OP, SAV, SK; Data Collection - OP, MAA, EBD; Writing - OP, SAV; Critical Revision - All of authors; Statistical Analysis -SK; Overall Responsibility - OP.

Conflict of Interest

The authors have no conflicts of interest to declare.

Financial Disclosure

The authors declare that this study has received no financial support.